The Agilent 2100 bioanalyzer is a highly successful microfluidics-based platform for the analysis of DNA, RNA, proteins and cells. This analytical instrument based on lab-on-a-chip technology the Agilent 2100 bioanalyzer delivers fast, automated, high quality digital data. It is used by us for DNA, RNA, Protein and whole cell work individually or in a typical workflow as follows:

Step 1: RNA isolation
The 2100 bioanalyzer utilizes the RNA6000 Labchip, the industry standard to control Total RNA, to perform many of the functions crucial to gene expression analysis through microarray technology.

• Eliminate steps and save time while using as little as 5 ng of total RNA

• Calculate the ratio of ribosomal bands in total RNA samples and show the percentage of ribosomal impurities in mRNA samples.

• Check the success of cRNA amplification reactions and insure sufficient sample.

Step 2: Gene expressions analysis
DNALabchips make it easy to get size and concentration information for each fragment of a sample.

• Check a PCR reaction faster and easier than ever.

• Quantify a template before a cloning experiment or sequencing reaction.

• Verify the outcome of an enzymatic digestion.

Step 3: Protein expression
Using the cell fluorescence and the cell assay extension,
our biochemists, molecular, protein and cell biologists can perform cell assays and simplify their workflow.

• Detect non-fluorescent, cell surface and intracellular proteins by antibody staining.

• Monitor transfection efficiency by Green Fluorescence Protein detection.

• Detect fluorescently labeled annexin-V for apoptosis.

 
Step 4: Protein purification

The 2100 bioanalyzer and the Protein Labchips are capable of analyzing a multitude of different protein samples. 

• Express recombinant proteins, purify proteins or work with antibodies.

• Identify impurities and control stability.

• Determine the absolute concentration of antibody preparations.