Home  ·  Product Search  ·  Checkout  ·  Track Your Order  ·  Site Map
Microbe Inotech Laboratories, Inc.

Repetitive Sequence-based PCR (repPCR)

Home
Contact Us
Who We Are
Our Services
Request Forms
Our Products
News & Media
Our Seminars
Privacy Policy
Our MiL Links

Cart

Items 0
Subtotal $0.00

Search by Keyword

Prokaryotic and eukaryotic genomes contain dispersed repetitive sequences separating longer single-copy DNA sequences.  Interspersed repetitive sequences are characterized as relatively short (usually <500 bp), non-coding, dispersed elements in bacterial genomes.  Current data supports that repetitive DNA comprises a substantial portion of the microbial genomes.  rep-PCR primers complement these repetitive sequences and allow for specific binding providing reproducible, unique rep-PCR DNA fingerprint patterns. 

 
Generation of Organism Specific rep-PCR DNA Fingerprint Patterns

1.  Rep-PCR primers bind to many specific repetitive sequences interspersed throughout the genome. 

  RepPCR Step 1

 

2. Multiple Fragments of various lengths are amplified

RepPCR Step 2

 

3.  Fragments can be separated by mass and charge via electrophoresis

RepPCR 3

 

4.  A unique rep-PCR DNA fingerprint profile created with multiple bands of varying intensity 

RepPCR Step 4

 


Software Overview   

  

Species and Subspecies of Mycobacterium                      Species and Subspecies of Mycobacterium

  

Copyright © Microbe Inotech Laboratories, Inc. 
7259 Lansdowne Avenue, Suite 200
St. Louis, MO 63119-3421
314.645.2177